Heavy Metal Adsorption (As, Zn, Pb, and Fe) using Indigenous Gram-Positive Bacteria from ASGM Wastewater in Lampung

Authors

  • Nuri Oktavia Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada and Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Hendra Prasetia Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Himawan Tri Bayu Murti Petrus Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada
  • Yekti Asih Purwestari Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada
  • Widi Astuti Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Fika Rofiek Mufakhir Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Aulia Afandi Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Asnan Rinovian Research Centre for Mineral Technology, National Research and Innovation Agency (BRIN)
  • Akhmad Subkhan Research Centre for Plantation Crop, Soekarno Science and Technology Park (KST)
  • Muhsin Wicaksono Albakri Research Center For Biotechnology, Universitas Gadjah Mada
  • Yuni Kusumastuti Chemical Engineering Department, Faculty of Engineering, Universitas Gadjah Mada

DOI:

https://doi.org/10.11113/bioprocessing.v5n1.91

Keywords:

ASGM, Heavy metals, Bioremediation, Micrococcus

Abstract

Small-scale gold mining (ASGM) produces toxic wastewater containing high concentration of heavy metals such as arsenic (As), lead (Pb), zinc (Zn), and iron (Fe) which threaten the environment and human health in the surrounding area. A biotechnological approach using metal-tolerant bacteria is an environmentally friendly alternative to physical and chemical methods. This study aims to test the ability of gram-positive bacteria, namely Micrococcus, isolated from ASGM tailings to absorb heavy metals As, Pb, Zn, and Fe. Isolation was carried out using Nutrient Agar (NA) and Nutrient Broth (NB) media, while tolerance tests were carried out at a concentration of 100 ppm for each metal. The results showed that Micrococcus bacteria could survive and resist exposure to contamination from the four heavy metals up to the highest concentration tested. These findings suggest that gram positive bacteria have significant potential for use as bioremediation agents in managing heavy metal pollution. In conclusion, Micrococcus can be used as an alternative in the remediation of As, Pb, Zn, and Fe metals, although further studies are still needed to evaluate their absorption capacity in more detail and their application in real environmental conditions.

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Published

2026-06-25

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