Physical Parameter Screening of Feather Protein Hydrolysate Production by Aspergillus terreus UniMAP AA-1 using Submerged Fermentation

Authors

  • Siti Nazeera Ahmad Zaki Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis, Perlis, Malaysia.
  • Syazwani Mahmad Puzi Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Arau, 02600, Perlis, MALAYSIA
  • Mohamad Fahrurrazi Tompang
  • Nurul Ain Harmiza Abdullah
  • Syazni Zainul Kamal Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis, Perlis, Malaysia
  • Zarina Zakaria
  • Huzairy Hassan
  • Sinar Arzuria Adnan
  • Nur Hidayah Ahmad Zaidi
  • Zulkarnain Mohamed Idris
  • Siti Khalijah Mahmad Rozi Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis, Perlis, Malaysia
  • Nur Izyan Wan Azelee
  • Mohd Nasrul Izzani Jamaludin Institute of Autonomous System, Universiti Teknologi Petronas, Perak, Malaysia

DOI:

https://doi.org/10.11113/bioprocessing.v3n2.43

Keywords:

Chicken feather, Protein hydrolysate, Submerged fermentation, Aspergillus terreus UniMAP AA-1, Fractional factorial design

Abstract

Feathers, a significant by-product of the poultry industry, pose considerable waste management challenges due to their abundance and resistance to degradation. This study explores the potential of utilizing chicken feather waste as a sustainable protein source through microbial fermentation. We employed Aspergillus terreus UniMAP AA-1, a keratinolytic fungus for fermentation aimed at degrading feather keratin. Various fermentation conditions, including initial pH, fermentation time, and inoculum size were tested using a fractional factorial design to optimize protein yield. The highest protein hydrolysate concentration achieved was 5.92 mg/mL, obtained with an inoculum size of 5%, a fermentation duration of 10 days, and an initial pH of 10.0. These results underscore the effectiveness of Aspergillus terreus UniMAP AA-1 in transforming feather waste into valuable protein hydrolysate. This research contributes to the advancement of eco-friendly and economically viable methods for valorizing poultry by-products, addressing environmental issues while providing innovative protein sources for livestock feed formulations.

References

Alamnie, G., Melake, A., Berhanu, Y., Alemu, M., Damtew, B., & Aemiro, A. (2024). Chicken Feather Protein Hydrolysate as a Low Cost Peptone Source for Microbial Cultivation: A Promising Perspective of Economic and Environmental Advantage. Case Studies in Chemical and Environmental Engineering, 9, 100741. https://doi.org/10.1016/j.cscee.2024.100741

Anas, N. A., & Arbain, D. (2012). Aspergillus terreus Unimap AA-1: A Newly Isolated Extracellular Glucose Oxidase-Producing Strain. World Applied Sciences Journal, 17(1), 86-90.

Grazziotin, A., Pimentel, F. A., de Jong, E. V., & Brandelli, A. (2006). Nutritional Improvement of Feather Protein by Treatment with Microbial Keratinase. Animal Feed Science and Technology, 126(1–2), 135–144. https://doi.org/10.1016/j.anifeedsci.2005.06.002

Grazziotin, Adriane, Pimentel, F. A., Jong, E. V. De, & Brandelli, A. (2008). Poultry Feather Hydrolysate as a Protein Source for Growing Rats. Brazilian Journal of Veterinary Research and Animal Science, 45(supl.), 61. https://doi.org/10.11606/S1413-95962008000700008

Gupta, R., & Ramnani, P. (2006). Microbial Keratinases and heir Prospective Applications: An Overview. Applied Microbiology and Biotechnology, 70(1), 21–33. https://doi.org/10.1007/s00253-005-0239-8

Kamarudin, N. B., Sharma, S., Gupta, A., Kee, C. G., Chik, S. M. S. B. T., & Gupta, R. (2017). Statistical Investigation of Extraction Parameters of Keratin from Chicken Feather using Design-Expert. 3 Biotech, 7(2), 127. https://doi.org/10.1007/s13205-017-0767-9

Kim, J. (2003). Keratinolytic Activity of FiveAspergillusSpecies Isolated from Poultry Farming Soil in Korea. Mycobiology, 31(3), 157. https://doi.org/10.4489/myco.2003.31.3.157

Khalel, A. (2020). Enhancing Plant Growth by Chicken Feather Compost Obtained from Feather Degradation by Streptomyces enissocaesili. Bioscience Biotechnology Research Communications, 13(4), 1847–1853. https://doi.org/10.21786/bbrc/13.4/32

Lowry O H, Rosebrough N J, F. A. L. & R. R. J. (2014). Citation Classics. In Encyclopedia of Quality of Life and Well-Being Research (Issue 1, pp. 921–921). Springer Netherlands. https://doi.org/10.1007/978-94-007-0753-5_100521

Martínez-Alvarez, O., Chamorro, S., & Brenes, A. (2015). Protein Hydrolysates from Animal Processing By-Products as a Source of Bioactive Molecules with Interest in Animal Feeding: A Review. Food Research International, 73(1069), 204–212. https://doi.org/10.1016/j.foodres.2015.04.005

Mazotto, A. M., Couri, S., Damaso, M. C., & Vermelho, A. B. (2013). Degradation of Feather Waste by Aspergillus Niger Keratinases: Comparison of Submerged and Solid-State Fermentation. International Biodeterioration & Biodegradation, 85, 189–195. https://doi.org/10.1016/j.ibiod.2013.07.003

Raveendran, S., Parameswaran, B., Ummalyma, S. B., Abraham, A., Mathew, A. K., Madhavan, A., Rebello, S., & Pandey, A. (2018). Applications of Microbial Enzymes in Food Industry. Food Technology and Biotechnology, 56(1), 16–30. https://doi.org/10.17113/ftb.56.01.18.5491

Rezende, C. A., Atta, B. W., Breitkreitz, M. C., Simister, R., Gomez, L. D. & McQueen-Mason, S. J. (2018). Optimization of Biomass Pretreatments using Fractional Factorial Experimental Design. Biotechnology for Biofuels, 11(206). https://doi.org/10.1186/s13068-018-1200-2

Riaz, A., Muzzamal, H., Maqsood, B., Naz, S., Latif, F. & Saleem, M. (2024). Characterization of a Bacterial Keratinolytic Protease for Effective Degradation of Chicken Feather Waste into Feather Protein Hydrolysates. Frontiers in Bioscience (Elite Ed) 16(3):22. https://doi.org/10.31083/j.fbe1603022

Saber, W. I. A., El-Metwall, M. M., & El-Hersh, M. S. (2010). Keratinase Production and Biodegradation of Some Keratinous Wastes by Alternaria tenuissima and Aspergillus nidulans. Research Journal of Microbiology, 5(1), 21–35. https://doi.org/10.3923/jm.2010.21.35

Sinkiewicz, I., Staroszczyk, H., & Śliwińska, A. (2018). Solubilization of Keratins and Functional Properties of Their Isolates and Hydrolysates. Journal of Food Biochemistry, 42(2), e12494. https://doi.org/10.1111/jfbc.12494

Sugiharto, S., & Ranjitkar, S. (2019). Recent Advances in Fermented Feeds towards Improved Broiler Chicken Performance, Gastrointestinal Tract Microecology and Immune Responses: A Review. Animal Nutrition, 5(1), 1–10. https://doi.org/10.1016/j.aninu.2018.11.001

Tesfaye, T., Sithole, B., & Ramjugernath, D. (2017). Valorisation of Chicken Feathers: A review on Recycling and Recovery Route—Current Status and Future Prospects. Clean Technologies and Environmental Policy, 19(10), 2363–2378. https://doi.org/10.1007/s10098-017-1443-9

Williams, C. M., Lee, C. G., Garlich, J. D., & Shih, J. C. H. (1991). Evaluation of a Bacterial Feather Fermentation Product, Feather-Lysate, as a Feed Protein. Poultry Science, 70(1), 85–94. https://doi.org/10.3382/ps.0700

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Published

2024-12-30

Issue

Vol. 3 No. 2: December 2024

Section

Articles