Optimizing Wall Material Ratios for Enhanced Bioactive Compound in Ficus deltoidea using Freeze Drying
DOI:
https://doi.org/10.11113/bioprocessing.v3n1.51Keywords:
ficus deltoidea, encapsulation, freeze dryAbstract
Ficus deltoidea (FD) is well documented for its pharmacological properties, including anticancer, antibacterial, anti-inflammatory, antiviral and anti-aging properties. However, the stability and adsorption of many bioactive compounds in herbs are poor, limiting their bioavailability. This study aimed to investigate the optimal formulation ratios and different wall materials (maltodextrin (MD) and gum Arabic (GA)) for encapsulating FD extract using freeze drying. Physicochemical characterizations of the formulated FD powders included evaluation of marker compound (isovitexin), phenolics (TPC) and flavonoids (TFC) content. The safety of the optimized encapsulated FD (OEFD) powder was assessed through microbiological assay. The optimal conditions, determined through response surface methodology (RSM), were a solid ratio of 20% w/v, a 75% MD: 25%GA and 3:1 of core to wall ratio, which resulted in the highest retention of isovitexin at 1.96 g/g, phenolic content of 1473 GAE mg/ 10 g extract and flavonoids content of 185 CE mg/ 10 g extract. Optimizing the freeze -drying process for FD extract improved its quality by retaining the marker compound. This optimization ensures that the bioactive compounds remain stable and effective, enhancing the therapeutic potential of the extract.
References
Abolmaesoomi, M., Aziz, A. A., Junit, S. M., & Ali, J. M. (2019). Ficus deltoidea: Effects of solvent polarity on antioxidant and anti-proliferative activities in breast and colon cancer cells. European Journal of Integrative Medicine, 28, 57–67.
Azmi, N. O. R. A. N. B. T. (2018). Virgin coconut oil based nanostructured lipid carrier loaded with ficus deltoidea extract for skin barrier improvement. Thesis Master of Phylosophy, Universiti teknologi Malaysia.
Choo, C. Y., Sulong, N. Y., Man, F., & Wong, T. W. (2012). Vitexin and isovitexin from the leaves of Ficus deltoidea with in-vivo α-glucosidase inhibition. Journal of Ethnopharmacology, 142(3), 776–781.
Farsi, E., Shafaei, A., Hor, S. Y., Ahamed, M. B. K., Yam, M. F., Attitalla, I. H., Asmawi, M. Z., & Ismail, Z. (2011). Correlation between enzymes inhibitory effects and antioxidant activities of standardized fractions of methanolic extract obtained from Ficus deltoidea leaves. African Journal of Biotechnology, 10(67), 15184–15194.
Heredia, J. F. A., Hernández-Carrión, M., Gómez-Franco, J. D., Narváez-Cuenca, C.-E., & del Pilar Sánchez-Camargo, A. (2024). Microwave-assisted extraction, encapsulation, and bioaccessibility of carotenoids from organic tomato industry by-product. Innovative Food Science & Emerging Technologies, 103706.
Husin, F., Khalid, N. N. M., Misran, E., Hasham, R., Hamid, M. A., & Ya’akob, H. (2024). In silico molecular docking study and in vitro evaluation of antioxidant activity in Kacip Fatimah. Materials Today: Proceedings.
Misbah, H., Aziz, A. A., & Aminudin, N. (2013). Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions. BMC Complementary and Alternative Medicine, 13(1), 118.
Munin, A., & Edwards-Lévy, F. (2011). Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics, 3(4), 793–829.
Shishir, M. R. I., & Chen, W. (2017). Trends of spray drying: A critical review on drying of fruit and vegetable juices. Trends in Food Science & Technology, 65, 49–67.
Soib, H. H., Ware, I., Yaakob, H., Mukrish, H., & Sarmidi, M. R. (2015). Antioxidant and anti-cancer actvity of standardized extracts of three varieties of Ficus deltoidea’s leaves. Jurnal Teknologi (Science & Engineering), 77(3), 19–25.
Tran, V. Van, Vu Nu, T. T., Hoang Bui, V. K., & Thi Tran, N. H. (2022). 10 - Use of herbal extract for body-care formulations. In S. H. Mohd Setapar, A. Ahmad, & M. B. T.-N. for the P. of C. U. P.-B. E. Jawaid (Eds.), Micro and Nano Technologies (pp. 263–282). Elsevier.
