Lactic Acid Production from Sequential Inorganic Salt Pretreated Oil Palm Empty Fruit Bunch via Simultaneous Saccharification and Fermentation
DOI:
https://doi.org/10.11113/bioprocessing.v1n1.6Keywords:
Lactic acid , Bacillus coagulans , Empty fruit bunch , Solid loading , Concentration CaCO3 , Enzyme loadingAbstract
Lactic acid is produced from inorganic salt pretreated oil palm empty fruit bunch (OPEFB) for the first time through simultaneous saccharification and fermentation (SSF). OPEFB is an agricultural waste that can be turned into lactic acid (LA), a highly desired chemical product. An inorganic salt (Na3PO4.12H2O-FeCl3) pretreatment precedes SSF with Bacillus coagulans DSM2314. The effect of solid loading, concentration CaCO3 and enzyme loading on LA generation is studied using design of experiments. The results show that solid loading and concentration CaCO3 affect LA yield. 50g/L biomass, 50g/L CaCO3 concentration, and 50 FPU/g cellulase enzyme yield maximal LA (46.66/L) and yield (0.93/g OPEFB). The model created to predict LA production was then validated.
References
Borshchevskaya, L. N., Gordeeva, T. L., Kalinina, A. N., & Sineokii, S. P. (2016). Spectrophotometric determination of lactic acid. Journal of Analytical Ahemistry, 71(8), 755-758.
Cunha, M. C. D., Masotti, M. T., Mondragón-Bernal, O. L., & Alves, J. G. L. F. (2018). Highly efficient production of L (+)-lactic acid using medium with potato, corn steep liquor and calcium carbonate by Lactobacillus rhamnosus ATCC 9595. Brazilian Journal of Chemical Engineering, 35, 887-900.
da Silva, D. B., Fernandes, B. S., & da Silva, A. J. (2021). Effect of initial pH and substrate concentration on the lactic acid production from cassava wastewater fermentation by an enriched culture of acidogenic microorganisms. Water Environment Research, 93(10), 1925-1933.
Hassan, N., & Idris, A. (2016). Simultaneous saccharification and fermentation of lactic acid from empty fruit bunch at high solids loading. BioResources, 11(2), 3799-3812.
Hassan, N., Anwar, N. A. K. K., & Idris, A. (2020). Strategy to Enhance the Sugar Production Using Recyclable Inorganic Salt for Pre Treatment of Oil Palm Empty Fruit Bunch (OPEFB). BioResources, 15(3), 4912-4931.
Koppram, R., & Olsson, L. (2012). Challenges and possibilities of second generation bioethanol production process at high substrate. In Science and Technology Day 2012, March 27, 2012, Göteborg, Sweden.
Martinez, F. A. C., Balciunas, E. M., Salgado, J. M., Gonzalez, J. M. D., Converti, A., & de Souza Oliveira, R. P. (2013). Lactic acid properties, applications and production: A review. Trends in Food Science & Technology, 30(1), 70-83.
Panesar, P. S., Kennedy, J. F., Gandhi, D. N., & Bunko, K. (2007). Bioutilisation of whey for lactic acid production. Food Chemistry, 105(1), 1-14.
Saavedra, S., Alejandro-Paredes, L., Flores-Santos, J. C., Flores-Fernández, C. N., Arellano-García, H., & Zavaleta, A. I. (2021). Optimization of lactic acid production by Lactobacillus plantarum strain Hui1 in a medium containing sugar cane molasses. Agronomía Colombiana, 39(1), 98-107.
Stenberg, K., Bollók, M., Réczey, K., Galbe, M., & Zacchi, G. (2000). Effect of substrate and cellulase concentration on simultaneous saccharification and fermentation of steam‐pretreated softwood for ethanol production. Biotechnology and Bioengineering, 68(2), 204-210.
van der Pol, E. C., Eggink, G., & Weusthuis, R. A. (2016). Production of L (+)-lactic acid from acid pretreated sugarcane bagasse using Bacillus coagulans DSM2314 in a simultaneous saccharification and fermentation strategy. Biotechnology for Biofuels, 9(1), 1-12.
Varga, E., Klinke, H. B., Réczey, K., & Thomsen, A. B. (2004). High solid simultaneous saccharification and fermentation of wet oxidised corn stover to ethanol. Biotechnology and Bioengineering, 88(5), 567-574.
Yang, P. B., Tian, Y., Wang, Q., & Cong, W. (2015). Effect of different types of calcium carbonate on the lactic acid fermentation performance of Lactobacillus lactis. Biochemical Engineering Journal, 98, 38-46.
