Carbohydrate Extraction from Brown Algae using Green Technology Approach - Ultrasonic Assisted Extraction (UAE)
DOI:
https://doi.org/10.11113/bioprocessing.v3n2.61Keywords:
Carbohydrate, Brown Algae, Green Technology, Ultrasonic-assisted Extraction (UAE)Abstract
Over the past decade, conventional methods have been widely employed for extracting carbohydrate from brown algae, involving significant quantities of acid and alkaline chemicals, lengthy processing times, and high energy requirements. However, in line with the current global emphasis on environmental friendliness, the focus has shifted towards green technologies in the extraction process. Green technologies usually refer to non-conventional methods, with Ultrasonic-Assisted Extraction (UAE) being one of the most commonly utilized techniques. This research paper focuses on the extraction of carbohydrates from brown algae using a green technology approach, specifically UAE. Central composite design (CCD) was used in this study with different combinations of parameters such as temperature (30-60 °C), time (15-45 min) and material to solvent ratio (1:15-1:35). The best operating parameters that obtained the highest carbohydrate yield are the material-to-solvent ratio of 1:35 g/mL, extraction time of 30 min, and extraction temperature of 45 °C. The highest yield of carbohydrate obtained was 7.73 mg/g. These findings will contribute to the development of sustainable and efficient methods for carbohydrate extraction from brown algae using green technology approaches.
References
Alboofetileh, M., Rezaei, M., Tabarsa, M., & You, S. 2019. Bioactivities of Nizamuddinia zanardinii Sulfated Polysaccharides Extracted by Enzyme, Ultrasound and Enzyme-Ultrasound Methods. Journal of Food Science and Technology, 56, 1212-1220.
García-Vaquero, M., Rajauria, G., O'Doherty, J. V., & Sweeney, T. 2017. Polysaccharides from Macroalgae: Recent Advances, Innovative Technologies and Challenges in Extraction and Purification. Food Research International, 99, 1011-1020.
Li, Y., Zheng, Y., Zhang, Y., Yang, Y., Wang, P., Imre, B., ... & Wang, D. 2021. Brown Algae Carbohydrates: Structures, Pharmaceutical Properties, and Research Challenges. Marine Drugs, 19(11), 620.
Nielsen, S. S. 2010. Phenol-sulfuric Acid Method for Total Carbohydrates. Food Analysis Laboratory Manual, 47-53.
Thao My, P. L., Sung, V. V., Dat, T. D., Nam, H. M., Phong, M. T., & Hieu, N. H. 2020. Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum Mcclurei and Testing Bioactivities of the Extract. ChemistrySelect, 5(14), 4371-4380.
Zhang, H., & Row, K. H. 2015. Extraction and Separation of Polysaccharides from Laminaria japonica by Size-Exclusion Chromatography. Journal of Chromatographic Science, 53(4), 498-502.
