Antibacterial and Antibiofilm Properties of Zingiber officinale and Kaempferia parviflora Against Staphylococcus aureus
DOI:
https://doi.org/10.11113/bioprocessing.v4n2.77Keywords:
Staphylococcus aureus, Zingiber officinale, Kaempferia parviflora, Antibacteria, AntibiofilmAbstract
Staphylococcus aureus is a pathogenic bacterium whose biofilm-forming ability contributes to resistance against antibacterial treatments, highlighting the need for alternative strategies using plant extracts. This study evaluated the antibacterial and antibiofilm properties of Zingiber officinale (normal ginger) and Kaempferia parviflora (black ginger) ethanolic extracts against S. aureus, focusing on extraction yield, total phenolic content (TPC), functional group characterization (ATR-FTIR) and their efficacy in inhibiting S. aureus growth (disc diffusion assay) and biofilm formation (biofilm assay). K. parviflora yielded 3.05% extract compared to 0.57% for Z. officinale but Z. officinale had a slightly higher TPC (43.03 mg GAE/100 g) compared to K. parviflora (39.83 mg GAE/100 g). ATR-FTIR analysis predicted the presence of phenols, alkane and aromatic functional group in both extracts. Antibacterial assay showed that only K. parviflora extract inhibited S. aureus, with an 8.33 mm zone of inhibition at the tested concentrations. For biofilm assay, K. parviflora extract effectively inhibited S. aureus biofilm formation after seven days, with the highest inhibition of 59.92%. Overall, K. parviflora demonstrated promising potential as a plant-based antibacterial and antibiofilm agent against S. aureus.
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